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Spectrophotometric method for monitoring the pH of tissue culture medium

✍ Scribed by Gerald J. Lancz; Timothe M. Chavez


Publisher
Springer
Year
1977
Tongue
English
Weight
311 KB
Volume
3
Category
Article
ISSN
1573-0603

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✍ Francesco Paoletti; Donatella Aldinucci; Alessandra Mocali; Anna Caparrini πŸ“‚ Article πŸ“… 1986 πŸ› Elsevier Science 🌐 English βš– 475 KB

Superoxide dismutase (EC 1.15.1.1) has been assayed by a spectrophotometric method based on the inhibition of a superoxide-driven NADH oxidation. The assay consists of a purely chemical reaction sequence which involves EDTA, Mn(II), mercaptoethanol, and molecular oxygen, requiring neither auxiliary