Spectrophotometric measurement of hydroxylamine and its O-alkyl derivatives

✍ Scribed by T.K. Korpela; Mauri J. Mäkelä

• Book ID: 102985644
• Publisher: Elsevier Science
• Year: 1981
• Tongue: English
• Weight: 543 KB
• Volume: 110
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(81)90143-3

✦ Synopsis

An easy spectrophotomettic method was developed to quantify compounds having an -O-NH* (amino-oxy) function (e.g., hydroxylamine, canaline, 0-aminoserine, and aminooxy acetic acid). Stoichiometric reactions occur, in practice, between the amino-oxy compounds and the aldehydic group of pyridoxal Y-phosphate in aqueous solution. When the reaction had reached equilibrium the concomitant decrease in absorption at 405 nm was used as the measure of the amino-oxy functions. Thus it is possible to determine amino-oxy compounds at about the same concentration as pyridoxal5'-phosphate can be measured spectrophotometrically. The present method was applied to follow the enzymic hydrolysis of canavanine to canahne. Based on the measured apparent kinetic constants, a specific way to determine hydroxylamine among its 0-alkylethers was advised, as well.