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Spectrophotometric flow-injection determination of ascorbic acid by generation of triiodide

✍ Scribed by J. Hernández-Méndez; A.Alonso Mateos; M.J.Almendral Parra; C.García De María


Publisher
Elsevier Science
Year
1986
Tongue
English
Weight
503 KB
Volume
184
Category
Article
ISSN
0003-2670

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✦ Synopsis


A method is proposed for the flow-injection determination of ascorbic acid (0.1-40 pg ml-'). Iodine is generated in the flow system as triiodide ion or the triiodide/starch complex giving a steady spectrophotometric signal at 350 or 580 nm, respectively; inverse peaks caused by ascorbic acid samples are measured. The method is applied to the determination of ascorbic acid in a fruit juice, jam and vitamin C preparation.


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A flow-injection spectrophotometric method for the determination of hydrazine is described. The method is based on the inhibitory effect of hydrazine on the reaction of thionine with nitrite in acidic media. The decolorization of thionine by the reaction with nitrite was used to monitor the reaction