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Spectrophotometric estimation of protein in presence of ultraviolet-absorbing impurities

โœ Scribed by C.W. Wrigley; H.L. Webster

Book ID
104145461
Publisher
Elsevier Science
Year
1968
Tongue
English
Weight
263 KB
Volume
33
Category
Article
ISSN
1873-3778

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โœฆ Synopsis

Several workers have recommended the spectrophotometric estimation of serum proteins at wavelengths in the region of 200-220 mp (TOMBS et aL.l, MURPHY AND 10~s~

). This communication emphasizes the specificity of this procedure for protein in presence of certain ultraviolet-absorbing impurities. The following experiment is presented as an example of the use of this procedure in the chromatographic fractionation of proteins from a crude tissue extract.

An extract of wheat leaves was prepared as described by WRIGLEY AND WEB-STERN and freed of low molecular weight compounds by passage through Sephadex G-50. When this preparation was fractionated by column chromatography on DEAEcellulose, a series of peaks (at fractions 14, 20 and 26

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A critical reappraisal of Waddell's tech
A critical reappraisal of Waddell's technique for ultraviolet spectrophotometric protein estimation
โœ Peter Wolf ๐Ÿ“‚ Article ๐Ÿ“… 1983 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 816 KB

Waddell's method of estimating protein concentration by the difference between spectrophotometric absorptions (2 15-225 nm) has been reexamined. Over limited ranges of total protein, a linear relation was found for ten purified proteins; the narrowest range was between 5 and 25 &ml. Using published