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Spectrophotometric determinations of traces of selenium by catalytic reduction of tetranitro blue tetrazolium

โœ Scribed by Wayne C. Hawkes

Publisher
Elsevier Science
Year
1986
Tongue
English
Weight
565 KB
Volume
183
Category
Article
ISSN
0003-2670

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โœฆ Synopsis

The method is based on the catalytic effect of selenium on the reduction of tetranitro blue tetrazolium by dithiothreitol; it is simple, rapid and effective with several forms of selenium. With this catalytic assay, 1.2 ng of Se as selenite, 21 ng of Se as selenocysteine or 150 ng of Se as selenocystine can be detected (3 X SD). The relative standard deviations of the method are 2.7%, 5.4%, and 9.1% for the determinations of 0.2 pg, 2 pg, and 5 fig Se as selenite, selenocysteine, and selenocystine, respectively. The method is free of interferences from common ions and most of the interfering cations can be masked with organic acids. Data are included on the effects of pH, temperature, and reagent concentrations.

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