Spectrophotometric Determination of Mimosine and 3-Hydroxy-4-(1H)-pyridone - The Toxic Principles of Leucaena leucocephala

✍ Scribed by K. Lalitha; C.M. Vargheese; N. Balasubramanian

• Publisher: Elsevier Science
• Year: 1993
• Tongue: English
• Weight: 493 KB
• Volume: 213
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1006/abio.1993.1385

✦ Synopsis

A sensitive and selective spectrophotometric method for the estimation of the toxic factors mimosine and 3hydroxy-4-(1 (H) )-pyridone (DHP) has been developed based on the intense yellow-colored azodye formed with p-nitroaniline which showed a sharp absorption maximum at (400 \mathrm{~nm}). The method was optimized based on relative sensitivity of the reaction with various aromatic primary amino compounds and under different conditions of (\mathrm{pH}). Interference from a variety of structurally related compounds and phenols was tested and found insensitive to this method. The molar extinction coefficient at (400 \mathrm{~nm}) for the azodye formed with mimosine was (5.31 \times 10^{4} \mathrm{M}^{-1} \mathrm{~cm}^{-1}) and that for DHP was (1.699 \times 10^{4} \mathrm{M}^{-1} \mathrm{~cm}^{-1}). The applicability of the method was tested using different plant extracts and recovery was found to be at (100 \pm 0.3 %). The method is suitable for accurate estimation of both mimosine and DHP after paper chromatographic separation of extracts of different biological samples. 1993 Academic Press, Inc.