Spectrophotometric determination of lantadene A, 22β-angeloxy-3-oxoolean-12-en-28-oic acid
✍ Scribed by O.P. Sharma; H.P.S. Makkar; R.K. Dawra
- Publisher
- Elsevier Science
- Year
- 1983
- Tongue
- English
- Weight
- 336 KB
- Volume
- 128
- Category
- Article
- ISSN
- 0003-2697
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✦ Synopsis
The formation of a blue chromogen between sodium borohydride-treated lantadene A (22@angeloyloxy-3-oxoolean-12-en-2%oic acid) and acetic anhydride-sulfuric acid (9: 1) formed the basis of a spectrophotometric method for its quantitation. The chromogen had a broad absorption maximum (X,,) at 630-645 nm. The optimum amount of sodium borohydride for lantadene A reduction was I mgjmg lantadene A in methanolic solution. The chromogen was stable for 5, 7, and 26 min after reaction at 25, 18, and O"C, respectively. The method is convenient, sensitive, and reproducible. The amount of lantadene A in the leaves of Lantana camara collected in the month of May quantitated by the present method was found to be 13.6 mp/g dry weight of the leaves.