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Spectrophotometric Determination of Deacetylation Degree of Chitinous Materials Dissolved in Phosphoric Acid

✍ Scribed by Hsien-Yi Hsiao; Chih-Cheng Tsai; Suming Chen; Bo-Chuan Hsieh; Richie L.C. Chen

Publisher: John Wiley and Sons
Year: 2004
Tongue: English
Weight: 83 KB
Volume: 4
Category: Article
ISSN: 1616-5187
DOI: 10.1002/mabi.200400084

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✦ Synopsis

Abstract

Summary: A simple spectrophotometric method is proposed for determining deacetylation degrees (DD) of chitinous materials using phosphoric acid as the UV‐transparent solvent system. Calibrating by the extinction coefficients (A~210~) of D‐glucosamine and N‐acetyl‐D‐glucosamine, DD values (24–88%) were computed numerically. The results correlated well (R^2^ = 0.9805, n = 50) with those obtained by solid‐state ^13^C NMR.

Comparison of the results obtained by the proposed UV method and solid‐state ^13^C NMR.

magnified imageComparison of the results obtained by the proposed UV method and solid‐state ^13^C NMR.

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