Spectrophotometric Determination of Ampicillin by Ternary Complex Formation with 1,10-Phenanthroline and Copper(II)

✍ Scribed by A.Z. Abuzuhri; A.H. Rady; M.S. Elshahawi; S.M. Aldhaheri

• Publisher: Elsevier Science
• Year: 1994
• Tongue: English
• Weight: 208 KB
• Volume: 50
• Category: Article
• ISSN: 0026-265X
• DOI: 10.1006/mchj.1994.1066

✦ Synopsis

A simple and convenient spectrophotometric procedure is described for the determination of ampicillin. The method is based on the formation of a yellow ternary complex with 1,10-phenanthroline and copper(II) in acetate buffer solution ((\mathrm{pH} \simeq 5.0)) and measurement at (446 \mathrm{~nm}). The stoichiometry and stability constant of the ternary complex were determined. Beer's law is obeyed in the concentration range (2.5-100 \mu \mathrm{gm}^{-3}). The optimum concentration range evaluated by Ringbom's method, the molar absorptivity, and the Sandell sensitivity of the ternary complex were determined. A variety of pharmaceutical dosage forms containing ampicillin were successfully analyzed by the proposed procedure, giving a relative standard deviation of (1.0 %). Comparison of results with those of an official method shows good agreement and indicates no significant difference in the precision of the two methods. 1994 Academic Press. Inc.