Spectrophotometric characteristics and assay of pure pyronin Y

✍ Scribed by P. Jakobsen; H. Lyon; S. Treppendahl

• Book ID: 104786775
• Publisher: Springer
• Year: 1984
• Tongue: English
• Weight: 294 KB
• Volume: 81
• Category: Article
• ISSN: 1432-119X
• DOI: 10.1007/bf00495408

✦ Synopsis

The spectrophotometric characteristics of analytically pure pyronin Y have been investigated. Addition of metal ions (Fe3+, Zn2+, and Mg2+) and of dextrin were shown not to influence the absorption characteristics. The composition of the solvent strongly influenced the value of the extinction coefficient. Aqueous ethanolic solutions with a content of about 50% ethanol gave higher epsilon-values than those found for more concentrated ethanol solutions. The difference can be explained by the existence of a solvent-solute complex in the less concentrated ethanol solutions. A new spectrophotometric assay is proposed using the epsilon-value 11.7 X 10(4) lmol-1 cm-1 found in aqueous ethanol (52%) as standard.