Spectrophotometric Assay ofd-Aspartate andd-Glutamate Usingd-Aspartate Oxidase with Malate Dehydrogenase and Glutamate Dehydrogenase
✍ Scribed by Mamoru Wakayama; Kazue Takashima; Yuko Tau; Sadatoshi Nakashima; Kenji Sakai; Mitsuaki Moriguchi
- Publisher
- Elsevier Science
- Year
- 1997
- Tongue
- English
- Weight
- 77 KB
- Volume
- 250
- Category
- Article
- ISSN
- 0003-2697
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✦ Synopsis
D-AO was prepared from the cell-free extract of F. sac-Spectrophotometric Assay of D-Aspartate chari Y-105 according to the purification method outlined and D-Glutamate Using D-Aspartate in a previous paper (9). One unit of D-AO was defined as Oxidase with Malate Dehydrogenase the amount of enzyme producing 1 mmol of keto acid and Glutamate Dehydrogenase per minute. MDH from yeast, GlDH from beef liver, and NADH were purchased from Oriental Yeast Co., Ltd. Protein concentration was estimated by the method of Lowry Mamoru Wakayama, Kazue Takashima, Yuko Tau, et al. (10). D-Asp and D-Glu were from Wako Pure Co., Sadatoshi Nakashima, Kenji Sakai, Ltd. All other chemicals were of analytical grade. and Mitsuaki Moriguchi 1
The assay systems for the coupling of D-AO with