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Spectrophotometric assay of yeast sterols using a polyene antibiotic

โœ Scribed by S.M. Rowe; W.J. Simpson; J.R.M. Hammond


Book ID
115271786
Publisher
John Wiley and Sons
Year
2008
Tongue
English
Weight
221 KB
Volume
13
Category
Article
ISSN
0266-8254

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A quick and simple affinity chromatography method for gauging the interaction of polyene antifungal agents with sterols has been developed. The required affinity columns are prepared from a standard C-18 reverse-phase HPLC column by injecting a measured quantity of sterol under conditions where it i