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Spectrophotometric assay of 2′,3′-cyclic nucleotide 3′-phosphohydrolase: Application to the enzyme in bovine brain

✍ Scribed by Tony E. Hugli; Michael Bustin; Stanford Moore


Book ID
118861127
Publisher
Elsevier Science
Year
1973
Tongue
English
Weight
669 KB
Volume
58
Category
Article
ISSN
0006-8993

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A new spectrophotometric assay for 2′,3′
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A new continuous spectrophotometric method for determining 2',3'-cyclic nucleotide 3'-phosphohydrolase (EC 3.1.4.37) is described. The assay method involves monitoring the decrease in pH which accompanies the hydrolysis of 2',3'-cyclic AMP. The reaction is performed in the presence of phenol red and