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Spectrophotometric analysis of sodium fluorescein aqueous solutions. Determination of molar absorption coefficient

✍ Scribed by M. C. Mota; P. Carvalho; J. Ramalho; E. Leite

Publisher: Springer Netherlands
Year: 1991
Tongue: English
Weight: 401 KB
Volume: 15
Category: Article
ISSN: 0165-5701
DOI: 10.1007/bf00128951

No coin nor oath required. For personal study only.

✦ Synopsis

The usefulness of sodium fluorescein (SF) and related physical parameters were analysed. Two factors that may affect the molar absorption coefficient (epsilon) of this compound were the presence of impurities and the pH of the solution. As discrepant values can be found in the literature for that coefficient, a purification technique was used and SF quantification was performed according to sodium concentration determined by atomic absorption spectrophotometry. The molar absorption coefficient of the SF solution in phosphate buffer pH 7.4 was determined. To study the influence of pH on epsilon determination, absorption spectra at pH1, 3, 5, and 10 were also analysed.

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