✦ LIBER ✦

Spectrophotometric Analysis of Carotenoids in Plant Extracts Based on Elimination of Chlorophyll Absorption

✍ Scribed by Mark N. Merzlyak; Inna Khozin; Zvi Cohen

Publisher: John Wiley and Sons
Year: 1996
Tongue: English
Weight: 468 KB
Volume: 7
Category: Article
ISSN: 0958-0344
DOI: 10.1002/(sici)1099-1565(199611)7:6<294::aid-pca317>3.0.co;2-o

No coin nor oath required. For personal study only.

✦ Synopsis

Subtraction of the absorption spectra of chlorophyll(s) from the total spectra of plant cell extracts reveals the carotenoid absorption spectra in both the ultraviolet and visible regions, from which the total carotenoid content can be easily evaluated. The applications of this method to Spirulina platensis cells treated with the herbicide norflurazon and to Anabaena siarnensis cells grown under high light irradiation are demonstrated. The possibility of subtraction of the absorption of both chlorophyll a and b is demonstrated in an extract of the leaves of a higher plant (grape).

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