Spectrophotofluorometric determination of salicylamide in blood serum and urine

✍ Scribed by Stephen A. Veresh; Foo Song Hom; John J. Miskel

• Publisher: John Wiley and Sons
• Year: 1971
• Tongue: English
• Weight: 441 KB
• Volume: 60
• Category: Article
• ISSN: 0022-3549
• DOI: 10.1002/jps.2600600723

✦ Synopsis

Of particular significance is the fact that diazoketone IVb is more active than ketol Vb which is derived from it. This strongly supports the assumption that the Clauberg activity measured for the diazoketones is a measure of their inherent activity and not simply a measure of the activity of their hydrolysis products.

It was also of interest to observe that the carboxylic acids IIIa-IIId showed a low, but real, Clauberg activity. The limited data available suggest that although these acids may not elicit a high level of response in this assay, up to a response level of 2.0+, their activity is equal to, or slightly greater than, that of the isomeric methyl esters. The calculated r-values ( 5 ) are also slightly greater for the acids than for the isomeric methyl esters.

None of the diazoketones has an activity so abnormal, either in comparison with the other members of this series or with that of the analogous methyl esters, as to suggest that it has alkylated the Clauberg receptor. However, the activity of diazoketones IVa, IVb, and, to a lesser extent, IVc seems surprisingly high, and it may warrant a reinvestigation with an assay based on intrauterine application of these compounds.