Spectrofluorometric Method for NO Determination

✍ Scribed by G. Gabor; N. Allon

• Book ID: 102559190
• Publisher: Elsevier Science
• Year: 1994
• Tongue: English
• Weight: 267 KB
• Volume: 220
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1006/abio.1994.1292

✦ Synopsis

A spectroscopic method for the quantitative determination of (\mathrm{NO}) in aqueous solution is presented. The method is based on the uv absorbance of nitrosothiols, coupled with the inner filter effect. A threshold of (5.10^{-7} \mathrm{M}) with a wide dynamic range of (5.10^{-7}-10^{-4} \mathrm{M}) are obtained. This method enables measurements in brain homogenates. Ultimately, since this method is immune to electrical perturbances, it will be applied at the tip of an optical fiber for in situ monitoring of NO or glutathione in brain and blood vessels. ic 1994 Academic Press, Inc.