β Scribed by F.A. Aly; A. El-Brashy; F. Belal
No coin nor oath required. For personal study only.
A rapid and highly sensitive fluorimetric procedure was developed for the routine determination of reserpine in bulk, in dosage forms and in biological fluids. The method is based on the fluorescence induced by oxidation of reserpine with 2-iodoxybenzoate in aqueous acetic acid. The oxidation product exhibits a greenish yellow fluorescence with its emission maximum at around 484 nm. The fluorescence intensity is a linear function of reserpine concentratiofi over the range 0.02-0.32 pg ml-' with a detection limit of 0.8 ng ml-'. The advantages and disadvantages of the proposed method are discussed and its applicability to different formulations and biological fluids is demonstrated.
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Recovery experiment. To determine the precision and accuracy of the above method, recovery experiments were performed using the method of additions. A fixed volume sample of solution was added to one of three different concentrations of the standard drug solution. The results are shown in Table 2.
## Abstract Yttriumβsensitized fluorescence was used to develop a sensitive and simple spectrofluorimetric method for the determination of sparfloxacin. The method is based on the strong fluorescence of sparfloxacin after adding the fluorescence probe yttrium in buffer solution (pH = 8), and variou