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Spectral measurements of large particles by flow cytometry

✍ Scribed by Dakota A. Watson; Daniel F. Gaskill; Leif O. Brown; Stephen K. Doorn; John P. Nolan

Publisher
John Wiley and Sons
Year
2009
Tongue
English
Weight
314 KB
Volume
75A
Category
Article
ISSN
0196-4763

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## Abstract Tumor necrosis factor‐α (TNF‐α) is a monokine of 17 kDa produced by activated macrophages and various cells involved in the immune system. We propose a new method for the measurement of TNF activity using flow cytometry. After an incubation with TNF, L929 cells were harvested and treate