Spectral assignments and anisotropy data of cellulose Iα: 13C-NMR chemical shift data of cellulose Iα determined by INADEQUATE and RAI techniques applied to uniformly 13C-labeled bacterial celluloses of different Gluconacetobacter xylinus strains
✍ Scribed by Stephanie Hesse-Ertelt; Raiker Witter; Anne S. Ulrich; Tetsuo Kondo; Thomas Heinze
- Book ID
- 102529680
- Publisher
- John Wiley and Sons
- Year
- 2008
- Tongue
- English
- Weight
- 299 KB
- Volume
- 46
- Category
- Article
- ISSN
- 0749-1581
- DOI
- 10.1002/mrc.2311
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✦ Synopsis
Abstract
Solid‐state ^13^C‐NMR spectroscopy was used to characterize native cellulose pellicles from two strains of Gluconacetobacter xylinus (ATCC 53582, ATCC 23769), which had been statically cultivated in Hestrin–Schramm (HS) medium containing fully ^13^C‐labeled β‐D‐glucose‐U‐^13^C~6~ as the sole source of carbon. For both samples, the ^13^C‐NMR chemical shifts were completely assigned for each ^13^C‐labeled site of cellulose I~α~ with the aid of 2D refocused INADEQUATE NMR. To determine the principal chemical shift tensor components, a pulse sequence based on the recoupling of anisotropy information (RAI) was applied at 10 kHz MAS. The detailed ^13^C tensors of cellulose I~α~ from different bacterial celluloses are thus available now for the first time, and these results have been compared with previously published data of nonenriched material and with theoretical predictions. Copyright © 2008 John Wiley & Sons, Ltd.