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Spectral analysis on the melting fine structure of λ DNA and T2 DNA

✍ Scribed by Chuji Akiyama; Osamu Gotoh; Akiyoshi Wada

Publisher
Wiley (John Wiley & Sons)
Year
1977
Tongue
English
Weight
411 KB
Volume
16
Category
Article
ISSN
0006-3525

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✦ Synopsis

Abstract

The fine structure of the thermal denaturation of viral DNA's was analyzed with the spectral method established by Felsenfeld and Hirschman, and Fresco et al. A fully automated system measuring melting curves at four wavelengths at the same time was developed for this purpose. With this device, (G + C)‐contents of melting components of λ DNA's were determined. The correlation between the (G + C)‐content and the melting temperature of each melting component is found to be well described by the linear relationship given by Marmur and Doty. The denaturation of each component is regarded as cooperative. The Marmur–Doty relation also stands for the local denaturation of T2 DNA, which has a narrow melting range.

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