✦ LIBER ✦
Spectral analysis of flow cytometric data: Design of a special-purpose low-pass digital filter
✍ Scribed by Peter M. A. Sloot; Pieter Tensen; Carl G. Figdor
- Publisher
- John Wiley and Sons
- Year
- 1987
- Tongue
- English
- Weight
- 555 KB
- Volume
- 8
- Category
- Article
- ISSN
- 0196-4763
No coin nor oath required. For personal study only.
✦ Synopsis
Spectral decomposition of flow cytometric datafiles of arbitrary dimension reveal infor
mation of both the signal and the noise cornponents that constitute the histograms. This spectral information is used to construct a low-pass digital filter, which removes the cal parameters. high-frequency noise from the actual data. It is shown that this procedure guarantees nontrivial smoothing of the flow cytometric data in accordance with the local experimental situation. Gs a consequence optimal reconstruction of the signal is possible, which facilitates unambiguous interpretation of the data files and mathematical estimation of the statisti-