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Specificity of lipoprotein lipase and hepatic lipase toward monoacylglycerols varying in the acyl composition

โœ Scribed by Craig H. Miller; J.Wallace Parce; Patricia Sisson; Moseley Waite


Book ID
115738295
Publisher
Elsevier Science
Year
1981
Tongue
English
Weight
848 KB
Volume
665
Category
Article
ISSN
0005-2760

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Lipoprotein lipase (LPL) and hepatic triglyceride lipase (HL) were biotinylated using N-hydroxysuccinamide ester of biotin (25-fold molar excess) which was incorporated into the lysine amino groups of the enzyme protein. By assessing enzyme activity and heparin-agarose affinity a biotinylation proto