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Specificity of cytochemical procedures for localising peroxidase activity in the sarcoplasmic reticulum

✍ Scribed by K. N. Christie; P. J. Stoward

Publisher: Springer
Year: 1979
Tongue: English
Weight: 796 KB
Volume: 64
Category: Article
ISSN: 1432-119X
DOI: 10.1007/bf00495032

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✦ Synopsis

Cytochemical evidence is reported for substantiating the view that when lightly-fixed skeletal muscle is incubated in a diaminobenzidine-H202 medium at pH 5, the resulting enhanced electron opacity of the sarcoplasmic reticulum is more likely to be due to a peroxidatic activity therein rather than to a non-enzymic binding reaction. The reticulum staining is absent in incubated sections of overfixed or boiled tissue; or if hydrogen peroxide is omitted from the incubation medium; or if aminotriazole is included in the medium.

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