Specific substrates for isolation and differentiation of Azotobacter vinelandii

✍ Scribed by Claus, D. ;Hempel, Waltraud

• Book ID: 104762428
• Publisher: Springer-Verlag
• Year: 1970
• Weight: 394 KB
• Volume: 73
• Category: Article
• ISSN: 0003-9276
• DOI: 10.1007/bf00409955

✦ Synopsis

Resorcinol, ethylene glycol and glutaric acid have been found to be specific substrates for the enrichment of Azotobacter vinelandii in the presence of other Azotobacter species. The three compounds may also be used for species differentiation in the genus Azotobacter. In contrast to other reports strains of A. chroococcum and A. bei]erinckil have been isolated which are able to use T.-rhamnose for growth.

Conditions for the production of a yellow pigment and green fluorescence by A. vinelandii from non-aromatic substrates on agar-plates have been tested.

The production of water soluble yellow pigments in benzoate containing media cannot be used as a criterium for the presence of A. vinelandii. The pigment seems to be cr semialdehyde, the splitting product resulting from rectacleavage of catechol. It may be formed by all Azotobacter species that can metabolize aromatic substrates.