Specific radioimmunoassay of glucitol-lysine — application to lens proteins in streptozotocin-diabetic rats

✍ Scribed by M. Yano; F. Hashimoto; M. Sato; C. Ohboshi; M. Hirota; I. Oshima; Y. Ohe; S. Shin; K. Shima

• Publisher: Springer
• Year: 1988
• Tongue: English
• Weight: 429 KB
• Volume: 31
• Category: Article
• ISSN: 0012-186X
• DOI: 10.1007/bf00290588

✦ Synopsis

A radioimmunoassay using antibody against glucitol-lysine was developed to quantitate glycated proteins in the lens of diabetic rats. The amount of glycated protein was expressed as molar equivalents of reduced glycated hippuryl lysine (GlcRED-Hip-Lysine). Significant differences (p less than 0.01) were found in the amounts of glycated protein in the lenses of rats with streptozotocin-induced diabetes (3.92 +/- 0.59 nmol/mg protein, n = 5), those with streptozotocin-induced diabetes treated with insulin (2.94 +/- 0.36 nmol/mg protein, n = 4) and normal rats (1.23 +/- 0.22 nmol/mg protein, n = 5). There was a significant correlation between the concentration of glycated protein in the lens and the HbA1c level at the end of the 12 week experiment (r = 0.957, p less than 0.001). These results indicate that glycation of lens protein is parallel with the severity of diabetes in rats.