Specific Radiation Damage Can Be Used to Solve Macromolecular Crystal Structures

Using X-ray absorption spectra to monito

Using X-ray absorption spectra to monitor specific radiation damage to anomalously scattering atoms in macromolecular crystallography

✍ Oliéric, V. ;Ennifar, E. ;Meents, A. ;Fleurant, M. ;Besnard, C. ;Pattison, P. ;S 📂 Article 📅 2007 🏛 International Union of Crystallography 🌐 English ⚖ 826 KB

Radiation damage in macromolecular crystals is not suppressed even at 90 K. This is particularly true for covalent bonds involving an anomalous scatterer (such as bromine) at the 'peak wavelength'. It is shown that a series of absorption spectra recorded on a brominated RNA faithfully monitor the ex