Specific PCR detection and identification ofXylella fastidiosastrains causing citrus variegated chlorosis

✍ Scribed by Margaret R. Pooler; John S. Hartung

• Book ID: 104709208
• Publisher: Springer
• Year: 1995
• Tongue: English
• Weight: 688 KB
• Volume: 31
• Category: Article
• ISSN: 0343-8651
• DOI: 10.1007/bf00294703

✦ Synopsis

By cloning and sequencing specific randomly amplified polymorphic DNA (RAPD) products, we have developed pairs of PCR primers that can be used to detect Xylella fastidiosa in general, and X. fastidiosa that cause citrus variegated chlorosis (CVC) specifically. We also identified a CVC-specific region of the X. fastidiosa genome that contains a 28-nucleotide insertion, and single base changes that distinguish CVC and grape X. fastidiosa strains. When using RAPD products to develop specific PCR primers, we found it most efficient to screen for size differences among RAPD products rather than presence/absence of a specific RAPD band.