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Specific cleavage of chicken αA-globin and human c-Ha-ras genes by two molecular forms of calf thymus topoisomerase I

✍ Scribed by I. B. Bronstein; I. I. Gromova; S. V. Razin


Publisher
Springer
Year
1991
Tongue
English
Weight
801 KB
Volume
101
Category
Article
ISSN
0300-8177

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✦ Synopsis


Two molecular forms of topoisomerase I differing in size and sensitivity to camptothecin were isolated from calf thymus. Mapping of topo I cleavage sites of the cloned chicken alpha A-globin and human c-Ha-ras genes was carried out. Camptothecin was shown to affect site specificity of the topoisomerases.