✦ LIBER ✦

Specific and rapid assay of urinary oxalic acid using high-performance liquid chromatography

✍ Scribed by Susumu Imaoka; Yoshihiko Funae; Toshikado Sugimoto; Nobuyuki Hayahara; Masanobu Maekawa

Publisher: Elsevier Science
Year: 1983
Tongue: English
Weight: 390 KB
Volume: 128
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(83)90401-3

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✦ Synopsis

A high-performance liquid chromatographic (HPLC) method was developed to determine the levels of oxalic acid in the urine. This acid was extracted from urine with tri-n-butyl phosphate and converted into the fluorescent derivative by esterification with 9-anthryldiazomethane (ADAM). The reaction mixture containing the oxalic acid derivative can be directly chromatographed on HPLC using octadecylsilane reverse-phase column monitoring with a fluorophotometric detector. A linear relationship was observed in the range from 1 to 100 &ml of standard oxalic acid dissolved in saline. Disease-free Japanese adults excrete 23.8 f 9.0 mg (mean + SD) of oxalic acid per day. This method should prove valuable for routine measurements of urinary oxalic acid as it is accurate, simple, and specific.

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