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Species-Specific in vitro Synthesis of DNA Containing the Polyoma Virus Origin of Replication

✍ Scribed by Yasufumi Murakami, Toshihiko Eki, Masa-Atsu Yamada, Carol Prives and Jerard Hurwitz

Book ID
123633394
Publisher
National Academy of Sciences
Year
1986
Tongue
English
Weight
958 KB
Volume
83
Category
Article
ISSN
0027-8424

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✍ NΓΌsslein-Crystalla, Volker ;Scheefers-Borchel, Ursula πŸ“‚ Article πŸ“… 1979 πŸ› Springer 🌐 English βš– 610 KB

The restriction nuclease cleavage pattern of E. coli DNA synthesized in vitro in the cellophane membrane system (Schaller et al., 1972) is similar to the one obtained after labelling E. coli in vivo. This is shown for exponentially growing cells and for cells synchronized by amino acid starvation fo