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Species identification of cyst and root-knot nematodes from potato by electrophoresis of individual females

✍ Scribed by Gerrit Karssen; Tine Van Hoenselaar; Brigitte Verkerk-Bakker; Richard Janssen

Publisher: John Wiley and Sons
Year: 1995
Tongue: English
Weight: 458 KB
Volume: 16
Category: Article
ISSN: 0173-0835
DOI: 10.1002/elps.1150160119

No coin nor oath required. For personal study only.

✦ Synopsis

Species identification of cyst and root-knot nematodes from potato by electrophoresis of individual females

To carry out rapid and reliable species identification of Globodera and Meloidogyne specimens, two electrophoretic methods were modified and adapted for an automated electrophoresis system (PhastSystem, Pharmacia). Proteins of individual Globodera cysts were identified using isoelectric focusing and a sensitive silver stain. Proteins of young single Meloidogyne females were separated using polyacrylamide gradient gels and stained for the isozymes esterase and malate dehydrogenase.

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