Speciation of vitamin B12 analogues by liquid chromatography with flame atomic absorption spectrometric detection

✍ Scribed by P. Viñas; N. Campillo; I.López García; M.Hernández Córdoba

• Publisher: Elsevier Science
• Year: 1996
• Tongue: English
• Weight: 615 KB
• Volume: 318
• Category: Article
• ISSN: 0003-2670
• DOI: 10.1016/0003-2670(95)00445-9

✦ Synopsis

Vitamin B,, analogues and aqueous cobalt are separated by liquid chromatography using a reversed-phase column (Spherisorb ODS-2) and a mobile phase consisting of a linear gradient from a 2575 (v/v) mixture of methanol and 0.085 M phosphoric acid solution buffered at pH 5.2 with triethanolamine to a 60:40 mixture over 5 min. The vitamins are selectively detected by measuring cobalt with an atomic absorption spectrometer directly interfaced to the chromatograph. A simple T-piece is used to compensate with air the difference between the nebulizer uptake rate and the chromatographic flow-rate (1.5 ml min-').

Recoveries of the components from spiked preparations ranged from 90.8 to 108%. The method is applicable to the analysis of pharmaceuticals for cobalamins.