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Spatially resolved fluorescence lifetime mapping of enzyme kinetics in living cells

✍ Scribed by V.K. RAMANUJAN; J.A. JO; G. CANTU; B.A. HERMAN

Book ID: 108866447
Publisher: John Wiley and Sons
Year: 2008
Tongue: English
Weight: 439 KB
Volume: 230
Category: Article
ISSN: 0022-2720
DOI: 10.1111/j.1365-2818.2008.01991.x

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## Background: The Cellscan mark-S (CS-S) scanning cytometer was used for tracing enzymatic reactions in the same individual cells under various physiological conditions over periods of minutes. On-line reagent addition and changes in the experimental conditions (buffers, ions, substrates and inhib