Spatially localized ballistic two-photon excitation in scattering media
✍ Scribed by Henryk Szmacinski; Ignacy Gryczynski; Joseph R. Lakowicz
- Publisher
- John Wiley and Sons
- Year
- 1998
- Tongue
- English
- Weight
- 217 KB
- Volume
- 4
- Category
- Article
- ISSN
- 1075-4261
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✦ Synopsis
We describe spatially localized two-photon excitation in scattering media. Using femtosecond pulses at 770 nm from a Ti : Sapphire laser, we were able to excite fluorophores in capillary tubes under up to 1.5 mm of 0.5% intralipid. Displacement of the laser beam relative to the embedded samples indicates that highly localized excitation was possible with two-photon excitation, whereas one-photon excitation resulted in loss of spatial resolution due to excitation by the diffusely scattered photons. These results indicate that two-photon excitation in the scattering solution is due only to the ballistic photons, a result confirmed by frequency-domain time-resolved measurements. Selective excitation of adjacent embedded samples was found possible for two but not one-photon excitation.