Spatial refractive index measurement of porcine artery using differential phase optical coherence microscopy
✍ Scribed by Jeehyun Kim; Digant P. Davé; Christopher G. Rylander; Junghwan Oh; Thomas E. Milner
- Publisher
- John Wiley and Sons
- Year
- 2006
- Tongue
- English
- Weight
- 217 KB
- Volume
- 38
- Category
- Article
- ISSN
- 0196-8092
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✦ Synopsis
Background and objectives:
We describe a methodology to record spatial variation of refractive index of porcine renal artery using differential phase optical coherence microscopy (dp-ocm).
Study design/materials and methods:
The dp-ocm provides quantitative measurement of thin specimen phase retardation and refractive index by measuring optical path-length changes on the order of a few nanometers and with a lateral resolution of 3 microm. the dp-ocm instrumentation is an all-fiber, dual-channel michelson interferometer constructed using a polarization maintaining (pm) fiber.
Results:
Two-dimensional en face dual-channel phase images are taken over a 150 x 200 microm region on a microscopic slide, and the images are reconstructed by plotting a two-dimensional refractive index map as the ocm beam is moved across the sample.
Conclusions:
Because the dp-ocm can record transient changes in the optical path-length, the system may be used to record quantitative optical path-length alterations of tissue in response to various stimuli. a fiber-based dp-ocm may have the potential to substantially improve in vivo imaging of individual cells for a variety of clinical diagnostics, and monitoring applications.