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Spatial heterogeneity of the metabolic response of RIF-1 tumors to a vasoactive agent evaluated in vivo by one-dimensional 31P chemical-shift imaging

✍ Scribed by Z. M. Bhujwalla; S. J. Blackband; J. P. Wehrle; J. D. Glickson


Publisher
John Wiley and Sons
Year
1992
Tongue
English
Weight
285 KB
Volume
26
Category
Article
ISSN
0740-3194

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✦ Synopsis


Abstract

Localized ^31^P NMR spectroscopy was used to evaluate the spatial heterogeneity of the metabolic response of RIF‐1 tumors to hydralazine. Volume localized ^31^P spectra were obtained from subcutaneous RIF‐1 tumors using one‐dimensional chemical‐shift imaging, before and 20 min after treatment with 5 mg/kg hydralazine, administered intravenously. Following treatment all of the tumors showed an overall decrease in the ratio of nucleoside triphosphate (NTP) to inorganic phosphate (P~i~) and a decrease in pH. However, spatial localization revealed that the reduction in NTP/P~i~ was not uniform within some tumors. This was partly due to regional differences in the levels of metabolites existing before treatment. Normal tissue adjacent to the tumor did not show a significant decrease in high‐energy metabolites or pH.