Spatial Characteristics of Cisterna Magna in Rats and Novel Technique for Puncture with a Stereotactic Manipulator

✍ Scribed by S. V. Lebedev; D. V. Blinov; S. V. Petrov

• Book ID: 111572873
• Publisher: Springer US
• Year: 2004
• Tongue: English
• Weight: 351 KB
• Volume: 137
• Category: Article
• ISSN: 0007-4888
• DOI: 10.1023/b:bebm.0000042732.00810.01

✦ Synopsis

The actual size and sites for puncture of the cisterna magna in adult Wistar rats were estimated on acrylic models. We proposed a new method for obtaining cerebrospinal fluid from narcotized rats. This method consists in controlled introduction of a needle into the cisterna magna through the skin and/or dura mater above this structure using a stereotactic micromanipulator. Experiments on rats with focal and global cerebral ischemia showed that this technique ensures single and repeated sampling of at least 50 µl liquor not containing blood admixtures.