๐”– Bobbio Scriptorium
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Sources of reducing power for nitrate reduction in spinach leaves

โœ Scribed by A. F. Mann; D. P. Hucklesby; E. J. Hewitt

Publisher
Springer-Verlag
Year
1978
Tongue
English
Weight
274 KB
Volume
140
Category
Article
ISSN
0032-0935

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โœฆ Synopsis

The possible source of NADH, the energy donor for nitrate reductase (EC 1.6.6.1), has been studied using an in vivo assay involving freezing the material (leaves of Spinacea oleracea L.) in liquid nitrogen in order to render the tissue permeable to added substrates. Glycolysis and the pentose phosphate pathway were capable of generating NADH through glyceraldehyde-3-phosphate dehydrogenase. Malate and isocitrate were also capable of generating NADH white other organic acids tested were not, including glycolate which was ineffective even under anaerobic conditions.

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The supply of reducing power for nitrite
The supply of reducing power for nitrite reduction in plastids of seedling pea roots (Pisum sativumL.)
โœ Michael J. Emes; Michael W. Fowler ๐Ÿ“‚ Article ๐Ÿ“… 1983 ๐Ÿ› Springer-Verlag ๐ŸŒ English โš– 590 KB

Plastids were separated from extracts of pea (Pisum sativum L.) roots by sucrose-densitygradient centrifugation. The incubation of roots of intact pea seedlings in solutions containing 10 mM KNO3 resulted in increased plastid activity of nitrite reductase and to a lesser extent glutamine synthetase.