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Sorting of cells from different cell cycle phases using surface antigen expression

✍ Scribed by Filippo Reno; Francesca Luchetti; Marco Vitale; Armando Gregorini; Massimo Valentini; Stefano Papa

Publisher
Springer
Year
1996
Tongue
English
Weight
494 KB
Volume
18
Category
Article
ISSN
1573-0603

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✦ Synopsis

Detailed procedures are presented for obtaining highly enriched human Daudi cell population in different cell cycle phases. This protocol allows harvest of enriched G~ or S-G2 phase cells based on the recognition of a cell surface marker antigen by anti-CD1 l a antibody and sorting. Sorted cells are able to cycle and a wave of synchronisation appears. Relative advantages of the method over chemically induced cell synchronisation are discussed.

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