Sonochemically prepared BSA microspheres containing Gemcitabine, and their potential application in renal cancer therapeutics
✍ Scribed by Olga Grinberg; Aharon Gedanken; Chitta Ranjan Patra; Sujata Patra; Priyabrata Mukherjee; Debabrata Mukhopadhyay
- Publisher
- Elsevier Science
- Year
- 2009
- Tongue
- English
- Weight
- 413 KB
- Volume
- 5
- Category
- Article
- ISSN
- 1742-7061
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✦ Synopsis
This report demonstrates the formation and characterization of sonochemically prepared bovine serum albumin (BSA)-Gemzar (Gemcitabine) microspheres and shows their increased anticancer activity compared to pristine Gemzar. The amount of loaded Gemzar was determined by light absorption measurements. The BSA-Gemzar composite was analyzed and characterized by optical microscopy and scanning electron microscopy. The release kinetics of Gemzar from the proteinaceous microspheres was tested. The BSA-Gemzar composite was examined for its anticancer activity (in vitro) in renal cancer cells (RCC, 786-O cells) using [(3)H]thymidine incorporation assays. It was found that the influence of the Gemzar-loaded microspheres on the cancer cells was significantly greater than that of an equimolar concentration of pristine Gemzar.