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Sonic Hedgehog (shh) expression in developing and regenerating axolotl limbs

✍ Scribed by Torok, Maureen A.; Gardiner, David M.; Izpis�a-Belmonte, Juan-Carlos; Bryant, Susan V.

Publisher
John Wiley and Sons
Year
1999
Tongue
English
Weight
480 KB
Volume
284
Category
Article
ISSN
0022-104X

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✦ Synopsis

Sonic hedgehog (shh) expression is detectable in the posterior mesenchyme of many developing vertebrate limbs. We have isolated an RT-PCR fragment from the axolotl, Ambystoma mexicanum, that has high identity to other vertebrate shh genes. We describe the localization of this transcript during development and regeneration and in response to tissue grafts and retinoic acid (RA) exposure in the axolotl. Even though axolotl digits show a reversed polarity of differentiation (anterior [A] to posterior [P]) when compared to other tetrapods (P to A), shh is nevertheless expressed on the posterior margin of developing and regenerating limb buds. When A cells are grafted adjacent to P cells, an ectopic domain of shh is induced. Exposure to retinoic acid (RA), a molecule known to alter pattern in all three limb axes in urodeles, results in ectopic expression of shh in anterior cells of the regeneration blastema. Prior to this induced expression in response to RA, there is an earlier response by the endogenous domain of shh, which is downregulated within the first few hours of exposure.

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