Some water-insoluble papain derivatives

✍ Scribed by Israel H. Silman; Martin Albu-Weissenberg; Ephraim Katchalski

• Book ID: 101713824
• Publisher: Wiley (John Wiley & Sons)
• Year: 1966
• Tongue: English
• Weight: 482 KB
• Volume: 4
• Category: Article
• ISSN: 0006-3525
• DOI: 10.1002/bip.1966.360040405

✦ Synopsis

A description is given of the preparation and pioperties of four water-insoluble papain derivatives by three different techniques: (a) covalent binding to a water-insoluble diazonium salt derived from a copolymer of p-aminophenylalanine and leucine; (b) binding to insoluble preparations of collagen via bisdiazobenzidine2,2'-disulfonic acid; (c) insolubilization by crosslinking the enzyme itself with bisdiazobenzidine. By all three methods it was possible to obtain stable water-insoluble papain derivatives retaining much of the activity of the native enzyme on low and high molecular weight substrates.