Some recombinant human cytokines stimulate glycosaminoglycan synthesis in human synovial fibroblast cultures and inhibit it in human articular cartilage cultures

✍ Scribed by Ilana Yaron; Frank A. Meyer; Jean-Michel Dayer; Ilan Bleiberg; Michael Yaron

• Publisher: John Wiley and Sons
• Year: 1989
• Tongue: English
• Weight: 670 KB
• Volume: 32
• Category: Article
• ISSN: 0004-3591
• DOI: 10.1002/anr.1780320210

✦ Synopsis

Recombinant human cytokines were compared for their effects on glycosaminoglycan (GAG) synthesis in human synovial fibroblast cultures and human articular cartilage explant cultures. In fibroblast cultures, recombinant human interleukin-la (rHuIL-la), rHuIL-lP, and recombinant human tumor necrosis factor a (rHuTNFa) stimulated hyaluronic acid (HA) production and, to a lesser extent, sulfated GAG production, while recombinant human y-interferon did not have a significant effect. Half-maximal stimulation of HA by rHuIL-lP was 0.14 pM, while stimulation for rHuIL-la and rHuTNFa was 1.6 pM and 32 pM, respectively. Indomethacin (10 pg/ml) had no influence on HA stimulation by cytokines, while hydrocortisone ( 2 1 0 pg/ml) caused a significant reduction. In articular cartilage cultures, the cytokines inhibited production of