Some Practical Aspects of Double-Resonance Techniques in Solution-State NMR Studies of High-Molecular-Weight Systems
✍ Scribed by Pearl Tsang; Mark Rance
- Publisher
- Elsevier Science
- Year
- 1996
- Tongue
- English
- Weight
- 233 KB
- Volume
- 111
- Category
- Article
- ISSN
- 1064-1866
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✦ Synopsis
This paper addresses some of the practical issues associated can be usefully applied (3-9); this has extremely important with solution NMR investigations of high-molecular-weight (ú20 consequences in terms of the range of biomolecules which kDa) biomolecules and their complexes. The experimental examcan be studied. With appropriate isotope-labeling schemes ple of a high-molecular-weight system used here is a 52 kDa Fab/ and multiple-resonance techniques, significant improvepeptide complex. Theoretical as well as experimental aspects of ments in spectral dispersion and/or editing can be achieved. solution NMR techniques employed to study such larger systems A more vexing problem which arises as the molecular are discussed. Specific topics covered include hardware considerasize increases is the concomitant lengthening of the reorientions, solvent suppression, and the relative efficiencies of various tational correlation time t c . As t c becomes longer, spinisotope-edited NMR techniques. Typical NOE methods used to
spin relaxation processes become increasingly more efficient derive structural information on these higher-molecular-weight and lead to a progressive broadening of the NMR resosystems and the scope of structural detail achievable are also examined.