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Some observations on the WATR method for water suppression at 80 MHz

✍ Scribed by Noel A. Dickinson; Ronald E. Lythgoe; Roger D. Waigh


Publisher
John Wiley and Sons
Year
1987
Tongue
English
Weight
194 KB
Volume
25
Category
Article
ISSN
0749-1581

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✦ Synopsis


The WATR method for water suppression in 'H NMR spectroscopy has been evaluated at 80MHz, using both ammonium and guanidinium chloride, with and without the addition of buffer salts. The pH of maximum effect has been found to depend on field strength and to be markedly affected by buffer salts, particularly phosphates. Provided that the pH is carefully controlled the method is simple and effective.

KEY WORDS 'H NMR WATR Water suppression Ammonium chloride Guanidinium chloride Buffer salts