Some morphogenic effects of sodium sulfate on tobacco callus

✍ Scribed by Stephen F. Chandler; Elena Ragolsky; Eng-Chong Pua; Trevor A. Thorpe

• Book ID: 104619618
• Publisher: Springer
• Year: 1987
• Tongue: English
• Weight: 453 KB
• Volume: 11
• Category: Article
• ISSN: 0167-6857
• DOI: 10.1007/bf00041847

✦ Synopsis

Callus cultures of Nicotiana tabacum L cv. Wisconsin 38 were initiated and grown on shoot-forming (SF) and callus proliferation (CP) medium with or without Na2SO 4. Two cultures were maintained on SF medium with 0, 0.75, 1 or 1.5% Na2SO 4 for 2.5 and 3.5 years. In the older culture only callus grown on salt formed shoots throughout the maintenance period, while in the younger culture the control responded best and Na 2 SO 4 was inhibitory. Callus from the older culture which had been grown on salt continued to form shoots in the absence of salt. Na 2 SO 4 caused adventitious shoot formation in three cultures on CP medium. These shoots were present for 7 subcultures after removal of Na2 SO4; but established, control callus, did not form shoots when transferred to Na 2 SOn. Callus initiated and maintained on NaC1 or mannitol showed a slight increase in shoot initiation. On NaC1, Na 2 SO4 or mannitol, the tissue osmotic potential became more negative and proline concentration increased.