Some effects of plant growth regulators on tissue cultures of the marine red algaAgardhiella subulata(Gigartinales, Rhodophyta)
✍ Scribed by Peter M. Bradley; Donald P. Cheney
- Publisher
- Springer
- Year
- 1990
- Tongue
- English
- Weight
- 568 KB
- Volume
- 204-205
- Category
- Article
- ISSN
- 1573-5141
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✦ Synopsis
We examined whether auxins and cytokinins, either singly or in combination, stimulate cell division in tissue cultures of a red seaweed . Our experimental model consisted of filamentous and callus-like growths that developed from cross-sectional discs cut from young branches of Agardhiella subulata . Plant growth regulators were added to the medium to give combinations of an auxin with a cytokinin over a range of concentrations (1 tg L -'-10 mg L -' ). Several mixtures of auxins and cytokinins, as well as some single auxins, cytokinins and phenolics, stimulated cell division and growth in the tissue cultures beyond that of controls . The treatments that were effective included : phenylacetic acid/zeatin ; phenylacetic acid/6-benzylaminopurine ; a-naphthaleneacetic acid/zeatin ; 2,4,5-trichlorophenoxyacetic acid/6-benzylaminopurine ; and indoleacetic acid/kinetin . High concentrations of cytokinins (i .e . 10 mg L -') inhibited the regeneration of plants in some of the cell cultures . These results provide further evidence that growth regulators can be used for the tissue culture of seaweeds and for the study of developmental phenomena in these plants .