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Some effects of pH and formaldehyde on the cellular electrokinetic surface

✍ Scribed by L. Weiss; Toni L. Cudney

Publisher
John Wiley and Sons
Year
1969
Tongue
French
Weight
561 KB
Volume
4
Category
Article
ISSN
0020-7136

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✦ Synopsis

When the electrophoretic mobilities of many cells are measured in solutions having p H values in excess of 9, or after formaldehyde-fixation, there is an increase in their net surface negativity which is usually attributed to deprotonation of amino-groups at the cell surface. A detailed investigation of the effects of high environmental p H and formaldehyde treatment on cell electrophoretic mobility suggests that the mobility changes are largely due to the adsorption of released intracrllular contents to the cell surface and that, contrary to current belief, information obtained by experiments of this type may not be interpreted to indicate the presence of positively-charged groups at the electrokinetic surfaces of intact living cells.

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