Some cytochemical characteristics of certain intranuclear inclusion bodies in the epithelium of the human vas deferens
✍ Scribed by Macdonald, John D.
- Publisher
- John Wiley and Sons
- Year
- 1950
- Tongue
- English
- Weight
- 949 KB
- Volume
- 106
- Category
- Article
- ISSN
- 0003-276X
No coin nor oath required. For personal study only.
✦ Synopsis
Intranuclear inclusion bodies in epithelial cells of the vas deferens and epididymis have been known since 1897, when Hammar observed them in the dog. Since then, surprisingly few investigators have devoted much attention to them. The few studies available are careful and detailed, but deal exclusively with the form and distribution of the inclusions; no cytochemical observations appear to have been published.
A few years ago many of these inclusion bodies were noticed in human material used in this department. At that time it was decided that a cytochemical study of them should be made. This paper is a result of that decision.
Methods
Materials used were specimens of human vas deferens obtained from surgical operations, including that in which the inclusions were first noted in this department. This specimen contained more inclusion bodies than any of the others. The case histories were available on 4 of the specimens.
For fixation the solutions of Bouin, Helly, Serra and Rossman (Lillie, '48) were used; also, the "Susa" mixture and I wish t o express m>sincere appreciation to Dr. Hadley Kirkman for his guidance and to Miss Cecil Kiinbrougli atid Mr. Frank Bnirett for their invaluable technical assistance.
327
Bismarck brown (Leach, '47) ; and thionine for metachromasia (Ballantyne, '29).
Whenever possible, control tissues were run with the specific staining reactions.
The following tissues were used:
KO. 15, from a 24-year-old individual. Epithelium of vas appeared to be abnormal in many places. Specimen of doubtful value. Fixed immediately after removal. Inclusions sparse.
KO.
16, from a 59-year-old individual. Vas removed to prevent complications of prostatectomy. Fixed immediately after removal. Kornial. Inclusions plentiful. Ko. 17, from a 38-year-old individual. Father of three childrcn. Fixed immediately after removal. Normal. Inclusions plentiful.
KO. 84G, from a 57-year-old individual. Doubly embedded by the celloidin-methyl benzoate method. Fixed two hours post-mortem. Inclusions abundant.
No. 84Ga, same as 84G, only embedded routinely in paraffin.
KO.
K259-1-data unavailable except that this specimeii was collected froin a
No. K259b-1 -data unavailable except that this specimen was collected from a KO. K259b-2data unavailable except that this specimen was collected from a young individual just past puberty. Inclusions Tery sparse or absent.
young individual just past puberty. Inclusions rery sparse or absent.
young individual just past puberty. Inclusions very sparse or absent.